U2OS Cells stably expressing Neurotensin Receptor NTSR1, Calcium Biosensor & β-arrestin biosensor

Description Gentaur Cells are cell lines stably co-expressing tag-free GPCRs and two Gentaur Biosensors to monitor G-protein and β-arrestin modulation. Each vial of Gentaur NTSR1 Neurotensin Receptor Cell Line contains U2OS cells stably expressing the following constructs: human NTSR1 Neurotensin Receptor with no tag Calcium Gentaur Biosensor (Green Fluorescence) β-arrestin Gentaur Biosensor (Red Fluorescence) Gentaur NTS1 Neurotensin Receptor Cell Line allows to assay compounds analyzing both signaling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to NTSR1 a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.